Despite the increase in the number of new businesses, significant barriers still exist for entrepreneurs.  This research examines barriers faced by business owners.  A survey to compare men and women business owners was conducted to determine if barriers for operating a business were different based on gender.  Results indicate significant differences between genders in several areas including legal knowledge, knowledge about financing operations, leadership skills, discrimination and creating technology infrastructure.  The mean for women was significantly higher in these areas compared to men which indicated higher levels of perceived barriers in these areas for women.